Milkweed’s bitter arms race against herbivores

ResearchBlogging.orgPlants are locked in a long twilight struggle with herbivores, particularly insects – sometimes they evolve a new defensive mechanism, “escaping” to diversify into new groups [$-a], but mostly natural selection works with the traits they already have. That means arms races – plants evolving greater concentrations of defense chemicals, and herbivores evolving greater tolerance of those chemicals. In this month’s Evolution, a new study of defensive chemistry evolution in milkweed [$-a] documents exactly this process.


Asclepias viridis, a milkweed
Photo by gravitywave.

The study by Agrawal et al. follows up on earlier work in the same group, which established the evolutionary relationships between the members of the milkweed genus, Asclepias. Milkweeds are named for their defense against insect herbivores, a milky sap full of nasty chemicals – coumaric acids, caffeic acids, cardenolides, and flavonoids. The authors raised a large sample of milkweed species in a controlled environment, then measured the levels of these chemicals in each species. By mapping the chemical profiles onto the previously-developed phylogeny of Asclepias, they could estimate how milkweeds’ chemistry has evolved since the genus first arose.


Aphids on Asclepias
Photo by aroid.

This analysis revealed that milkweeds have gotten nastier over their evolutionary history. But it’s not that clear-cut: the diversity of defensive chemicals present in Asclepias decreased, even as the total production increased – so the plants seemed to be paring down an initial diversity of defenses into a few chemicals that worked especially well. Coumaric and caffeic acids, which are produced from the same biochemical precursors, forced a trade-off so that as one increased, the other decreased. On the other hand, cardenolides and flavonoids, which are both produced in another biochemical pathway, were positively associated.

If this sounds complicated, that’s because it is. As Agrawal and his coauthors point out, we actually don’t have a good sense at what timescale an arms race should manifest – that is, are we talking about plants evolving greater defenses over a few generations, or over millions of years, as this study? Natural selection can appear to be moving a population strongly in one direction for a year or two – and then turn out to be fluctuating all over the place [$-a] if you watch for decades. How year-to-year selection acting on multiple traits translates into the grand trends of evolution – whether the explosive diversification of flowering plants or the emergence of human intelligence – remains one of the big puzzles for those of us who study the living world.

Reference

A.A. Agrawal, J.-P. Salminen, M. Fishbein (2009). Phylogenetic trends in phenolic metabolism of milkweeds (Asclepias): Evidence for escalation. Evolution, 63 (3), 663-73 DOI: 10.1111/j.1558-5646.2008.00573.x

P.R. Ehrlich, P.H. Raven (1964). Butterflies and plants: a study in coevolution Evolution, 18, 586-608 DOI: http://www.jstor.org/pss/2406212

P.R. Grant, B.R. Grant (2002). Unpredictable evolution in a 30-Year study of Darwin’s finches Science, 296 (5568), 707-11 DOI: 10.1126/science.1070315

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Against specialist herbivores, plants give up

Plants put up with a lot – everyone wants to eat them! And, basically, there are two ways a plant might respond to being eaten. They can put energy into regrowing bits that get eaten, or they can put energy into making a lot of some nasty chemical, like the milky sap in milkweed. The trouble with the first option is obvious – it doesn’t do anything to stop the damage. But the trouble with the second is that, whenever plants evolve a new defensive strategy, herbivores evolve a way around it. Often, these herbivores do very well, because they can eat something no one else can – and they become specialists on their new favorite food.


Photo by Melete.

Evolutionary ecologists have been thinking about this plant-herbivore arms race ever since Darwin. Back in 1964, Paul Erhlich and Peter Raven proposed that plants and insects might go through alternating cycles of diversification [$-a] driven by the evolution of new plant defenses and insect counterdefenses. Now, in a new paper in last week’s PNAS, Anurag A. Agrawal (who is at the top of everyone’s reference list) and Mark Fishbein show that sometimes, plants just throw in the towel [$-a].

Agrawal and Fishbein examine the evolutionary history of milkweed, which has a number of interesting anti-herbivore defenses besides the eponymous sap – and a number of specialized herbivores, like the red milkweed beetle pictured here. Their analysis looks for long-term evolutionary trends in the degree to which milkweeds put their energy into defenses, and the degree to which they put energy into regrowth. Over evolutionary time, it seems that milkweeds have reduced their defenses, and increased their regrowth efforts.

References

A. A. Agrawal, M. Fishbein (2008). Phylogenetic escalation and decline of plant defense strategies PNAS, 105 (29), 10057-10060 DOI: 10.1073/pnas.0802368105

P.R. Ehrlich, P.H. Raven (1964). Butterflies and plants: A study in coevolution Evolution, 18 (4), 586-608

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